November 23, 2009, 10:27 am PhpLabWare version 0.62

--Links-- Valelab Google Pubmed --Databases-- RNAi lib v.1 RNAi lib v.2 RNAi primers MitoSpindleScreen MitoSpindleScreen Plates MitoSpindleScreen Rows --System-- my settings logout     Now Viewing Table: MitoSpindleScreen View: test2 compact poles remarks Plate-normalized Row-normalized roy-hits Gohta GFP Localization             43 Records found. Showing 1 through 43.  Records per page             No Yes + - Anaphase-like Anstral --Clear --Likely --Weak Defective overall --Clear --Likely --Weak Dim g-tub --Clear --Likely --Weak Dim MT --Clear --Likely --Weak g-tub-like --Clear --Likely Long spindle --Clear --Likely --Weak Misalign --Clear --Likely --Weak Monastral bipolar --Clear --Likely --Weak Monopolar --Clear --Likely --Weak Multipolar --Clear --Weak Out of focus Pole detachment --Clear --Likely --Weak Short spindle --Clear --Likely --Weak Unfocused spindle MTs --Clear --Likely --Weak multipolar gtub area monopolar short spindles long spindles dim gtub in poles large nuc area high nuc num high circ2num pole detach dist pole detach number pole detached half/cell     name symbol CG plate RNAi probe hit cdc27 RNAi manual hits computer hits remarks galleries GFP localization Action microspherule protein 1 (nucleolar, ribosome gene regulation)-GG CG1135 1135 1 probe info Yes + Dim g-tub Dim g-tub - Clear dim gtub in poles Spindle morphology looks ok g-tubulin staining is dimmer - Clear 1 2   Ribosomal protein L19 RpL19 2746 7 probe info No +       1 2 3   Dim1, or thioredoxin-like U5 snRNP protein U5-15kD _GG Dim1 3058 9 probe info Yes + Misalign Misalign - Clear long spindles large nuc area Misalign - Clear 1 2   Mov34 (Proteasome, 26S subunit, non-ATPase -GG) Mov34 3416 11 probe info Yes + Monastral bipolar - Clear Monastral bipolar   monastral bipolar - Clear Few mitotic - Clear 1   U2 small nuclear riboprotein auxiliary factor 38 U2af38 3582 12 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear Monastral bipolar - Likely Monastral bipolar high nuc num Misalign - Clear monastral bipolar - Likely large spindle - Clear 1   unknown -GG CG5543 5543 23 probe info No + Misalign Misalign - Likely large nuc area Misalign - Likely 1   Kin17, antigenic determinant of recA protein -GG kin17 5649 24 probe info No + Misalign Misalign - Likely Monastral bipolar - Weak Monastral bipolar   monastral bipolar - Weak Misalign - Likely The phenotypes were not detected in additional repeat experiments. 1   squamous-cell carcinoma T-cell-recognized antigen -GG CG6686 6686 30 probe info No + Long spindle Long spindle - Likely   large spindle - Likely Few mitotic - Clear 1   abnormal spindle asp 6875 31 probe info Yes + Unfocused spindle MTs Pole detachment Unfocused spindle MTs - Clear Pole detachment - Clear long spindles dim gtub in poles high nuc num pole detached half/cell This must be Asp RNAi - Clear Unfocused spindle MTs - Clear 1 2 3   SAK/Plk4 - GG SAK 7186 33 probe info Yes + Monastral bipolar - Clear Anastral Anastral - Clear Monastral bipolar monopolar anastral - Clear monastral bipolar - Clear 1 2 3   RNA polymerase II 33kD subunit RpII33 7885 38 probe info Yes + Long spindle Long spindle - Likely   Few mitotic - Likely large spindle - Likely 1   Myb oncogene-like Myb 9045 45 probe info Yes + Long spindle Long spindle - Clear Monastral bipolar - Clear Monastral bipolar   Few mitotic - Likely monastral bipolar - Clear large spindle - Clear 1 2   Ndc80 -GG CG9938 9938 50 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles Misalign - Clear large spindle - Clear Few mitotic - Weak Thinner spindle - Likely 1 2   Sas4 -GG CG10061 10061 50 probe info Yes + Monastral bipolar - Likely Anastral Anastral - Weak Monastral bipolar   monastral bipolar - Likely anastral - Weak 1 2 3 4   RNA binding motif protein 22 -GG CG14641 14641 67 probe info No + Long spindle Long spindle - Likely Misalign Misalign - Weak   Misalign - Weak large spindle - Likely Few mitotic - Weak The phenotypes were not detected in additional repeat experiments. 1 2   Dgt1 -GG CG18041 18041 74 probe info Yes + Dim g-tub Dim g-tub - Weak dim gtub in poles Dimmer g-tub staining - Weak 1 2   Calmodulin Cam 8472 80 probe info Yes + Unfocused spindle MTs Long spindle Long spindle - Likely Monastral bipolar - Clear g-tub-like g-tub-like - Likely Unfocused spindle MTs - Clear Monastral bipolar long spindles large nuc area pole detach number pole detached half/cell Unfocused spindle MTs - Clear g-tub-like - Likely large spindle - Likely monastral bipolar - Clear 1 2 3 4   Ribosomal protein L11 RpL11 7726 81 probe info Yes + Short spindle Short spindle - Clear short spindles small spindle - Clear Few mitotic - Clear 1   similar to calmodulin regulated spectrin-associated protein 1-like 1 -GG l(2)k07433 33130 81 probe info Yes +   short spindles   1 2 3 4   unknown -GG l(2)08717 15095 81 probe info Yes + Long spindle - Weak Long spindle Misalign Misalign - Clear long spindles large spindle - Weak Misalign - Clear 1 2 3 4   Minute(3)62F (ribosomal protein L12e -GG) M(3)62F 3195 82 probe info Yes + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1   Dgrip163 -GG Grip163 5688 84 probe info Yes + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Likely   Dimmer g-tub staining - Likely large spindle - Likely 1 2   chromosome bows (Mast/Orbit/CLASP -GG) chb 32435 85 probe info Yes + Monopolar - Clear Monopolar monopolar I guess this is Mast/Orbit [CLASP] - Normal Monopolar - Clear 1 2 3 4   jumeau jumu 4029 86 probe info Yes + Long spindle Long spindle - Likely Misalign Anaphase-like Misalign - Clear large nuc area I also feel there are many anaphase-like cells - Likely large spindle - Likely Misalign - Clear 1 2   Sas-6 -GG CG15524 15524 89 probe info Yes +     This gene turned out to be Sas6, centriole duplication gene that should produce anastral or MAB spindles However, I still cannot see such defects here. The reason is that day4 is not long enough - I had the same problem before and had anastral phenotype at day 6 or 7. Therefore I mark this genes as a hit. 1 2 3   Nuf2? - GG CG8902 8902 97 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear large nuc area high nuc num large spindle - Clear Misalign - Clear 1 2 3   unknown -GG CG31887 31887 98 probe info No + Long spindle Long spindle - Likely Misalign Misalign - Likely   large spindle - Likely Misalign - Likely 1 2 3 4   unknown -GG CG9305 9305 100 probe info No + Long spindle Long spindle - Clear Misalign Misalign - Clear   large spindle - Clear Misalign - Clear The phenotypes were not detected in additional repeat experiments. 1 2 3 4   unknown -GG CG31763 31763 100 probe info No + Monopolar - Weak Monopolar Short spindle Short spindle - Likely   Monopolar - Weak small spindle - Likely This gene is not marked as a hit, because off-target-effect is possible: the RNAi probes have total six 16-bp overlaps with other short-spindle-producing genes (CG3992/SRP and CG18397). 1 2 3   Dgt2 -GG CG16969 16969 100 probe info Yes + Long spindle - Weak Dim g-tub Long spindle Dim g-tub - Clear dim gtub in poles large spindle - Weak Dimmer g-tub staining - Clear 1 2 3   Ana2 -GG CG8262 8262 105 probe info Yes + Monastral bipolar - Weak Monastral bipolar   monastral bipolar - Weak This RNAi was accidentally repeated using 150D1 probe, and I marked "Anastral Likely" and "Monastral bipolar Weak". So, reproducible phenotypes. 1 2   unknown -GG CG30026 30026 106 probe info No + Dim g-tub Dim g-tub - Clear dim gtub in poles Dimmer g-tub staining - Clear This gene is not marked as a hit, because I think this is off-target-effect of the probe: the probe very likely also targets a known gamma-tubulin-binding protein Dgrip91 (CG10988) that has four 16-bp identical sequences. GFP-CG30026 result also supports this notion. 1 2 3 4   Dgt5 -GG CG8828 8828 107 probe info Yes + Long spindle Long spindle - Clear   Large spindle - Clear 1 2 3   Cuticle protein LPCP-23 precursor -GG CG7548 7548 117 probe info No + Long spindle Long spindle - Clear Misalign Misalign - Clear large nuc area Large spindle - Clear Misalign - Clear The phenotypes were not detected in additional repeat experiments. 1 2 3   Mis12? -GG CG18156 18156 117 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles high nuc num large spindle - Clear Misalign - Clear 1 2 3 4   CENP-C -GG CG31258 31258 127 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear Pole detachment Pole detachment - Likely high circ2num Pole detachment - Likely Large spindle - Clear Few mitotic - Likely Misalign - Clear 1   dalmatian dmt 8374 127 probe info Yes + Long spindle Long spindle - Likely Misalign Misalign - Clear large nuc area Large spindle - Likely Misalign - Clear Few mitotic - Weak 1 2   Cal2/Spc25? - GG CG7242 7242 129 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles high circ2num Large spindle - Clear Misalign - Clear 1 2 3   Cal1 -GG CG5148 5148 131 probe info Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear   Misalign - Clear Large spindle - Clear 1 2 3   Ana1 -GG CG6631 6631 134 probe info Yes + Monastral bipolar - Clear Anastral Anastral - Clear Monastral bipolar monopolar Monastral bipolar - Clear Anastral - Clear 1 2 3 4   Dgt6 -GG CG11881 11881 136 probe info Yes + Dim g-tub Long spindle Long spindle - Clear Dim g-tub - Clear pole detach number Astral MTs look longer and more abundant. - Clear g-tub on the spindle is very dim. - Clear Dimmer g-tub - Clear Large spindle - Clear 1 2 3 4   unknown -GG CG14781 14781 138 probe info Yes + Monastral bipolar - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Likely Monastral bipolar   Monastral bipolar - Clear Monopolar - Clear Small spindle - Likely 1 2 3   Dgt4 -GG CG4865 4865 139 probe info Yes + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Clear   g-tub is dim on the spindle. - Likely Dimmer g-tub - Likely Astral MTs look longer and abundant. - Clear Large spindle - Clear 1 2 3 4