November 23, 2009, 10:30 am PhpLabWare version 0.62

--Links-- Valelab Google Pubmed --Databases-- RNAi lib v.1 RNAi lib v.2 RNAi primers MitoSpindleScreen MitoSpindleScreen Plates MitoSpindleScreen Rows --System-- my settings logout     Now Viewing Table: MitoSpindleScreen View: test2 compact poles remarks Plate-normalized Row-normalized roy-hits Gohta GFP Localization             205 Records found. Showing 1 through 205.  Records per page             No Yes No Yes No Yes + - Anaphase-like Anstral --Clear --Likely --Weak Defective overall --Clear --Likely --Weak Dim g-tub --Clear --Likely --Weak Dim MT --Clear --Likely --Weak g-tub-like --Clear --Likely Long spindle --Clear --Likely --Weak Misalign --Clear --Likely --Weak Monastral bipolar --Clear --Likely --Weak Monopolar --Clear --Likely --Weak Multipolar --Clear --Weak Out of focus Pole detachment --Clear --Likely --Weak Short spindle --Clear --Likely --Weak Unfocused spindle MTs --Clear --Likely --Weak multipolar gtub area monopolar short spindles long spindles dim gtub in poles large nuc area high nuc num high circ2num pole detach dist pole detach number pole detached half/cell     name symbol CG plate row col RNAi probe repeats cells/image mitotic index hit re-imaged positive control cdc27 RNAi manual hits computer hits remarks galleries GFP localization Action RheB -GG Rheb 1081 1 C 4 probe info Repeat 54.71 2.57% Yes   No + Misalign Misalign - Likely   Misalign - Likely Few mitotic - Likely 1     microspherule protein 1 (nucleolar, ribosome gene regulation)-GG CG1135 1135 1 E 12 probe info Repeat 166.12 2.41% Yes   No + Dim g-tub Dim g-tub - Clear dim gtub in poles Spindle morphology looks ok g-tubulin staining is dimmer - Clear 1 2   RNA polymerase II 18kD subunit RpII18 1163 86 A 9 probe info Repeat 65.08 5.59% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Likely   Misalign - Likely large spindle - Clear Few mitotic - Weak 1     Heat shock protein 83 Hsp83 1242 1 H 3 probe info Repeat 27.31 2.88% Yes   No + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Clear long spindles dim gtub in poles Dimmer g-tubulin staining - Likely large spindle - Clear Few mitotic - Clear 1     snRNP D2-GG CG1249 1249 1 H 8 probe info Repeat 59.48 1.12% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear   large spindle - Clear Misalign - Clear Few mitotic - Clear 1     pavarotti pav 1258 1 H 11 probe info Repeat 49.57 5.48% Yes   No + Multipolar Multipolar - Clear multipolar gtub area large nuc area DNA masses are larger - Likely Multipolar - Clear Brighter MT staining - Clear 1     Ribosomal protein L8 RpL8 1263 1 H 12 probe info Repeat 67.64 0.56% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     snRNP U2 -GG U2A 1406 2 E 6 probe info Repeat 67.81 2.00% Yes   No + Misalign Misalign - Clear   Misalign - Clear Few mitotic - Likely 1     step II splicing factor SLU7 -GG CG1420 1420 2 F 4 probe info Repeat 69.07 2.21% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Clear   large spindle - Likely Misalign - Clear Few mitotic - Likely 1     Klp10A/Kinesin-13 Klp10A 1453 2 G 9 probe info Repeat 135.48 8.79% Yes   Yes + Long spindle Long spindle - Likely long spindles high nuc num pole detach dist large spindle - Likely 1 2 3 4     no distributive disjunction nod 1763 4 D 5 probe info Repeat 89.71 7.14% Yes   Yes + Misalign Misalign - Likely   Misalign - Likely 1 2     Ribosomal protein L31 RpL31 1821 79 H 8 probe info Repeat 95.63 1.19% Yes     + Dim MT Monopolar - Clear Monopolar Dim MT - Likely Short spindle Short spindle - Clear   Small spindle - Clear Dimmer MT - Likely Monopolar - Clear Few mitotic - Clear 1     CAP-D2 subunit of condensin -GG CAP-D2 1911 5 B 11 probe info Repeat 114.50 5.55% Yes   Yes + Misalign Misalign - Clear   Chromatin condensation is defective - Clear Misalign - Clear 1 2 3     &agr;-Tubulin at 84B &agr;Tub84B 1913 5 C 1 probe info Repeat 84.43 10.58% Yes   Yes + Dim MT Dim MT - Clear Monopolar - Clear Monopolar monopolar Monopolar - Clear Dimmer MT staining - Clear 1 2 3 4 5     dynein light intermediate chain -GG Dlic2 1938 5 C 8 probe info Repeat 112.96 7.89% Yes   Yes + Pole detachment Pole detachment - Likely long spindles Pole detachment - Likely 1 2 3 4     Ubiquitin conjugating enzyme UbcD6 2013 86 A 6 probe info Repeat 133.33 4.67% Yes   No + Monastral bipolar - Likely Monopolar - Weak Monopolar Monastral bipolar   monastral bipolar - Likely Monopolar - Weak 1 2     Ribosomal protein S15Aa RpS15Aa 2033 90 G 6 probe info Repeat 134.17 1.81% Yes   No + Short spindle Short spindle - Weak   small spindle - Weak Few mitotic - Weak 1     Ribosomal protein L35A RpL35A 2099 86 B 3 probe info Repeat 32.39 1.29% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Ribosomal protein S3A RpS3A 2168 6 E 8 probe info Repeat 151.00 0.68% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Elongation factor 2b Ef2b 2238 6 G 9 probe info Repeat 270.40 3.45% Yes   No + Short spindle Short spindle - Weak short spindles small spindle - Weak 1 2 3     Pre-mRNA splicing factor CWC25 -GG CG2843 2843 8 B 7 probe info Repeat 116.43 4.52% Yes   No + Misalign Misalign - Weak long spindles Misalign - Weak 1 2     noisette (Splicing factor 3a, subunit 3 -GG) noi 2925 8 F 1 probe info Repeat 70.87 0.47% Yes   No + Misalign Misalign - Likely   Misalign - Likely Few mitotic - Clear 1     Dim1, or thioredoxin-like U5 snRNP protein U5-15kD _GG Dim1 3058 9 D 12 probe info Repeat 136.07 4.51% Yes   No + Misalign Misalign - Clear long spindles large nuc area Misalign - Clear 1 2   &ggr;-Tubulin at 23C &ggr;Tub23C 3157 9 H 12 probe info Repeat 161.73 6.45% Yes   Yes + g-tub-like g-tub-like - Clear monopolar long spindles dim gtub in poles g-tub-like - Clear 1 2 3     RNA polymerase II 140kD subunit RpII140 3180 10 B 4 probe info Repeat 63.67 3.40% Yes   No + Long spindle Long spindle - Likely long spindles large spindle - Likely Few mitotic - Clear 1     Minute(3)62F (ribosomal protein L12e -GG) M(3)62F 3195 82 G 4 probe info Repeat 105.94 0.76% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1   Ribosomal protein L17 RpL17 3203 90 B 6 probe info Repeat 70.19 1.36% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Clear 1     Dgt3 -GG CG3221 3221 111 F 5 probe info Repeat 108.03 14.25% Yes     + Long spindle Long spindle - Clear   Large spindle - Clear 1 2 3     male-specific lethal 2 (dosage compensation regulator -GG) msl-2 3241 96 D 1 probe info Repeat 194.53 8.55% Yes   No + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Likely Misalign Misalign - Likely monopolar dim gtub in poles pole detached half/cell Dimmer g-tub signals - Likely Misalign - Likely large spindle - Likely 1 2 3 4     EB1 -GG Eb1 3265 10 E 10 probe info Repeat 222.27 6.93% Yes   Yes +   short spindles dim gtub in poles Knowing that this is EB1 afterwards, the phenotype makes sense. I initially wondered a bit whether I should add a flag on this, since I rocognised small and round spindles. I should have added the flag!! 1 2 3 4     RNA polymerase II 15kD subunit RpII15 3284 10 F 11 probe info Repeat 166.30 4.17% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Weak long spindles high nuc num high circ2num large spindle - Weak 1 2     Ribosomal protein L7A RpL7A 3314 10 H 4 probe info Repeat 129.30 0.77% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Ribosomal protein S9 RpS9 3395 84 C 12 probe info Repeat 35.69 1.09% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Mov34 (Proteasome, 26S subunit, non-ATPase -GG) Mov34 3416 11 D 7 probe info Repeat 50.67 1.64% Yes   No + Monastral bipolar - Clear Monastral bipolar   monastral bipolar - Clear Few mitotic - Clear 1   twilight (Myb-interacting protein 130 -GG) twit 3480 11 G 3 probe info Repeat 205.83 4.73% Yes   No + Long spindle Long spindle - Likely Monastral bipolar - Weak Monastral bipolar high nuc num high circ2num pole detach number large spindle - Likely monastral bipolar - Weak 1 2     U2 small nuclear riboprotein auxiliary factor 38 U2af38 3582 12 C 3 probe info Repeat 66.17 5.24% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear Monastral bipolar - Likely Monastral bipolar high nuc num Misalign - Clear monastral bipolar - Likely large spindle - Clear 1   Ribosomal protein L23 RpL23 3661 82 D 10 probe info Repeat 34.11 1.95% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Spliceosomal protein on the X Spx 3780 13 D 11 probe info Repeat 114.00 1.37% Yes   No + Long spindle Long spindle - Likely   large spindle - Likely Few mitotic - Clear 1     gamma-tubulin ring protein 84 Grip84 3917 14 B 6 probe info Repeat 108.87 8.08% Yes   Yes + g-tub-like g-tub-like - Clear   g-tub-like - Clear 1 2 3     serpent (GATA-like TF -GG) srp 3992 14 F 4 probe info Repeat 87.80 1.56% Yes   No + Dim MT Dim MT - Clear Monopolar - Clear Monopolar   Dimmer MT staining - Clear Monopolar - Clear Few mitotic - Likely 1     Ribosomal protein L39 RpL39 3997 113 E 6 probe info Repeat 69.23 0.96% Yes     + Misalign Misalign - Likely Monopolar - Likely Monopolar Short spindle Short spindle - Clear   Misalign - Likely Monopolar - Likely Small spindle - Clear Few mitotic - Clear 1     Rad21 cohesin -GG Rad21 4008 72 D 12 probe info Repeat 49.92 6.91% Yes   Yes + Misalign Misalign - Likely   Misalign - Likely Few mitotic - Weak 1 2     jumeau jumu 4029 86 F 4 probe info Repeat 123.72 4.67% Yes   No + Long spindle Long spindle - Likely Misalign Anaphase-like Misalign - Clear large nuc area I also feel there are many anaphase-like cells - Likely large spindle - Likely Misalign - Clear 1 2   Eukaryotic initiation factor 4E eIF-4E 4035 14 H 4 probe info Repeat 219.40 3.51% Yes   No + Short spindle Short spindle - Clear short spindles dim gtub in poles small spindle - Clear 1 2     Ribosomal protein S16 RpS16 4046 82 C 9 probe info Repeat 56.03 0.74% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     similar to ribosomal protein L35e CG4111 4111 15 D 4 probe info Repeat 83.07 3.13% Yes   No + Defective overall - Likely Defective overall Short spindle Short spindle - Clear   Defective overall - Likely small spindle - Clear Few mitotic - Likely 1     Eukaryotic initiation factor 2&bgr; eIF-2&bgr; 4153 15 E 12 probe info Repeat 73.30 1.46% Yes   No + Defective overall - Weak Defective overall   Few mitotic - Clear Defective overall - Weak 1     26S proteasome non-ATPase regulatory subunit 8 -GG Rpn12 4157 15 F 3 probe info Repeat 33.23 1.60% Yes   No + Monopolar - Likely Monopolar   Monopolar - Likely Few mitotic - Clear 1     Regulatory associated protein of mTOR (Raptor) -GG CG4320 4320 16 E 6 probe info Repeat 119.17 7.53% Yes   No + Misalign Misalign - Clear dim gtub in poles Misalign - Clear 1 2 3     Borealin Bor 4454 98 G 1 probe info Repeat 59.47 2.19% Yes   Yes + Defective overall Multipolar Monopolar - Likely Multipolar - Clear Monopolar Defective overall - Clear   Defective overall - Clear Monopolar - Likely Multipolar - Clear Few mitotic - Clear 1     SUMO -GG smt3 4494 17 E 6 probe info Repeat 101.33 0.27% Yes   No + Defective overall Defective overall - Clear   Defective overall - Clear Few mitotic - Clear 1     Ribosomal protein L13 RpL13 4651 18 E 7 probe info Repeat 126.78 0.74% Yes   No + Unfocused spindle MTs Short spindle Short spindle - Clear Unfocused spindle MTs - Likely   Unfocused spindle MTs - Likely small spindle - Clear Few mitotic - Clear 1     DP transcription factor Dp 4654 18 E 9 probe info Repeat 77.14 5.87% Yes   No + Long spindle Long spindle - Likely long spindles high circ2num large spindle - Likely Brighter MT staining - Clear 1 2     ribosomal protein L27e -GG CG4759 4759 19 B 11 probe info Repeat 96.70 0.69% Yes   No + Monopolar - Clear Monopolar monopolar Monopolar - Clear Few mitotic - Clear 1     centrosomin cnn 4832 107 H 11 probe info Repeat 160.52 5.01% Yes   Yes + Anastral Anastral - Clear   This phenotype is very similar to centrosomin, maybe centrosomin itself. - Clear Anastral - Clear 1 2     Ribosomal protein L3 RpL3 4863 19 H 2 probe info Repeat 143.29 5.81% Yes Yes   + Monopolar - Clear Monopolar Short spindle Short spindle - Likely   Few mitotic - Likely Monopolar - Clear Small spindle - Likely 1     Dgt4 -GG CG4865 4865 139 E 4 probe info Repeat 159.12 12.54% Yes     + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Clear   g-tub is dim on the spindle. - Likely Dimmer g-tub - Likely Astral MTs look longer and abundant. - Clear Large spindle - Clear 1 2 3 4   Ribosomal protein L7 RpL7 4897 20 A 6 probe info Repeat 104.60 0.57% Yes   No + Monastral bipolar - Likely Short spindle Short spindle - Clear Monastral bipolar   small spindle - Clear monastral bipolar - Likely Few mitotic - Clear 1     mini spindles msps 5000 20 G 4 probe info Repeat 203.27 6.02% Yes   Yes + Monastral bipolar - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Clear Monastral bipolar gtub area monopolar monastral bipolar - Clear Monopolar - Clear small spindle - Clear - - - 1 2 3     Cal1 -GG CG5148 5148 131 A 7 probe info Repeat 89.20 15.36% Yes     + Long spindle Long spindle - Clear Misalign Misalign - Clear   Misalign - Clear Large spindle - Clear 1 2 3   Proteasome 25kD subunit Pros25 5266 87 A 2 probe info Repeat 46.72 9.85% Yes     + Dim g-tub Monastral bipolar - Likely Monopolar - Likely Monopolar Dim g-tub - Weak Monastral bipolar   Dimmer g-tub - Weak Monastral bipolar - Likely Monopolar - Likely 1     Proteasome 26S subunit subunit 4 ATPase Pros26.4 5289 22 D 12 probe info Repeat 47.93 0.56% Yes   No + Monastral bipolar - Likely Monopolar - Likely Monopolar Monastral bipolar   monastral bipolar - Likely Monopolar - Likely Few mitotic - Clear 1     Small ribonucleoprotein particle protein B SmB 5352 22 G 5 probe info Repeat 100.00 0.70% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Clear   Misalign - Clear large spindle - Likely Few mitotic - Clear 1     Tcp1-like T-cp1 5374 22 H 7 probe info Repeat 163.40 10.20% Yes   Yes + Dim MT Dim MT - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Clear short spindles dim gtub in poles Monopolar - Clear small spindle - Clear Dimmer MT staining - Clear 1 2 3 4     RNA polymerase III 39 kDa polypeptide F -GG CG5380 5380 22 H 11 probe info Repeat 174.40 6.27% Yes   No + Misalign Misalign - Likely high circ2num Misalign - Likely 1 2 3     snRNP U1 -GG CG5454 5454 23 D 8 probe info Repeat 65.97 1.11% Yes   No +   large nuc area Few mitotic - Clear 1     TCP-1 chaperone CG5525 5525 23 G 12 probe info Repeat 111.50 7.26% Yes   Yes + Dim MT Dim MT - Clear Monopolar - Clear Monopolar dim gtub in poles Monopolar - Clear Dimmer MT staining - Clear 1 2 3     Protein phosphatase 1 at 87B Pp1-87B 5650 24 E 10 probe info Repeat 117.63 6.55% Yes   Yes + Short spindle Short spindle - Weak   small spindle - Weak 1 2     Dgrip163 -GG Grip163 5688 84 F 7 probe info Repeat 55.56 11.05% Yes   Yes + Dim g-tub Dim g-tub - Likely Long spindle Long spindle - Likely   Dimmer g-tub staining - Likely large spindle - Likely 1 2   three rows thr 5785 110 A 12 probe info Repeat 54.07 2.34% Yes     + Defective overall - Likely Long spindle Long spindle - Likely Defective overall Misalign Misalign - Likely   Large spindle - Likely Chromatin structure is abnormal. - Clear Defective overall - Likely Misalign - Likely Few mitotic - Likely 1     Ribosomal protein L37A RpL37A 5827 176 G 1   Repeat 82.93 1.49% Yes     + Dim MT Dim MT - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Clear   Monopolar - Clear Small spindle - Clear Dimmer MT - Clear Few mitotic - Clear 1     DNA replication-related element factor Dref 5838 99 A 7 probe info Repeat 82.83 12.64% Yes   No + Misalign Misalign - Weak long spindles Misalign - Weak The phenotypes were not detected in additional repeat experiments. 1 2     Partial similarity to fruitless/TF -GG CG6118 6118 130 C 8 probe info Repeat 120.51 8.45% Yes   No + Dim g-tub Dim g-tub - Weak gtub area dim gtub in poles Dimmer g-tub - Weak 1 2 3 4     Ribosomal protein L9 RpL9 6141 77 G 3 probe info Repeat 72.67 1.24% Yes     + Monopolar - Likely Monopolar Short spindle Short spindle - Clear   Monopolar - Likely Small spindle - Clear Few mitotic - Clear 1     Dgrip75 -GG Grip75 6176 27 E 12 probe info Repeat 207.25 3.54% Yes   Yes + Dim g-tub Long spindle Long spindle - Likely Dim g-tub - Weak dim gtub in poles large spindle - Likely I feel that g-tub signals are dimmer 1 2 3     Ribosomal protein L14 RpL14 6253 84 C 3 probe info Repeat 36.53 1.14% Yes   No + Monopolar - Likely Monopolar Short spindle Short spindle - Likely   small spindle - Likely Monopolar - Likely Few mitotic - Clear 1     CENP-meta cmet 6392 28 H 8 probe info Repeat 142.39 2.50% Yes   Yes + Misalign Misalign - Weak   Misalign - Weak 1 2     Ribosomal protein L18A RpL18A 6510 29 F 2 probe info Repeat 66.11 0.50% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Clear 1     endosulfine endos 6513 120 F 11 probe info Repeat 112.50 14.79% Yes     + Long spindle Long spindle - Likely Misalign Misalign - Clear   Large spindle - Likely Misalign - Clear 1 2 3 4     Brahma associated protein 55kD Bap55 6546 29 G 9 probe info Repeat 49.19 3.73% Yes   No + Defective overall - Likely Defective overall   Few mitotic - Clear Defective overall - Likely 1     IplI-aurora-like kinase ial 6620 30 C 1 probe info Repeat 40.97 0.81% Yes   Yes +     Few mitotic - Clear 1     Ana1 -GG CG6631 6631 134 B 11 probe info Repeat 99.33 9.64% Yes   No + Monastral bipolar - Clear Anastral Anastral - Clear Monastral bipolar monopolar Monastral bipolar - Clear Anastral - Clear 1 2 3 4   RNA polymerase II 13.3 kDa polypeptide -GG Rpb11 6840 78 D 11 probe info Repeat 139.00 7.57% Yes     + Dim g-tub Long spindle Long spindle - Likely Dim g-tub - Weak   Large spindle - Likely Dimmer g-tub - Weak 1 2     ribosomal protein L26 -GG CG6846 6846 31 F 12 probe info Repeat 71.56 0.31% Yes   No +   monopolar Few mitotic - Clear 1     abnormal spindle asp 6875 31 H 5 probe info Repeat 132.56 5.32% Yes   Yes + Unfocused spindle MTs Pole detachment Unfocused spindle MTs - Clear Pole detachment - Clear long spindles dim gtub in poles high nuc num pole detached half/cell This must be Asp RNAi - Clear Unfocused spindle MTs - Clear 1 2 3   Vacuolar H -transporting two-sector ATPase, C subunit -GG VhaPPA1-1 7007 87 C 10 probe info Repeat 75.72 5.34% Yes     + Long spindle - Weak Long spindle Pole detachment Pole detachment - Likely   Large spindle - Weak Pole detachment - Likely 1     TCP1, subunit 2 (beta) -GG CG7033 7033 32 H 6 probe info Repeat 114.61 9.82% Yes   Yes + Dim g-tub Dim MT Dim MT - Clear Monopolar - Likely Monopolar Dim g-tub - Weak Short spindle Short spindle - Clear dim gtub in poles Monopolar - Likely small spindle - Clear Dimmer MT staining - Clear g-tub staining is also dimmer - Weak 1 2 3 4     SAK/Plk4 - GG SAK 7186 33 H 4 probe info Repeat 158.31 5.09% Yes   Yes + Monastral bipolar - Clear Anastral Anastral - Clear Monastral bipolar monopolar anastral - Clear monastral bipolar - Clear 1 2 3   Cal2/Spc25? - GG CG7242 7242 129 A 7 probe info Repeat 112.86 9.14% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles high circ2num Large spindle - Clear Misalign - Clear 1 2 3   Helicase at 25E Hel25E 7269 34 D 6 probe info Repeat 47.51 2.60% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear dim gtub in poles large nuc area high nuc num large spindle - Clear Misalign - Clear Few mitotic - Weak 1     ribosomal protein L10Ae -GG CG7283 7283 34 E 2 probe info Repeat 66.96 0.35% Yes   No + Monopolar - Likely Monopolar   Monopolar - Likely Few mitotic - Clear 1     Ribosomal protein L36A RpL36A 7424 77 C 1 probe info Repeat 72.23 1.02% Yes     + Monopolar - Clear Monopolar Short spindle Short spindle - Clear   Monopolar - Clear Small spindle - Clear Few mitotic - Clear 1     Ribosomal protein L22 RpL22 7434 35 D 1 probe info Repeat 82.33 5.74% Yes   No + Short spindle Short spindle - Weak   small spindle - Weak 1 2     Dynein heavy chain 64C Dhc64C 7507 35 G 12 probe info Repeat 105.22 8.71% Yes   Yes + Pole detachment - Weak Pole detachment long spindles pole detach number pole detached half/cell Pole detachment - Weak 1 2 3     Bub3 kinetochore protein -GG Bub3 7581 36 C 5 probe info Repeat 82.79 1.51% Yes   No + Misalign Misalign - Likely   Misalign - Likely Few mitotic - Weak 1     Proteasome 54kD subunit Pros54 7619 36 E 1 probe info Repeat 69.64 4.69% Yes   No + Short spindle Short spindle - Likely short spindles small spindle - Likely 1 2 3     Transcription factor -GG Spt5 7626 36 E 5 probe info Repeat 77.61 4.85% Yes   No + Misalign Misalign - Likely   Misalign - Likely 1 2 3     Ribosomal protein L11 RpL11 7726 81 G 1 probe info Repeat 37.67 1.40% Yes   No + Short spindle Short spindle - Clear short spindles small spindle - Clear Few mitotic - Clear 1   proteasome 26S non-ATPase subunit 2 -GG Rpn1 7762 37 C 4 probe info Repeat 36.96 1.24% Yes   No + Monastral bipolar - Likely Monastral bipolar   monastral bipolar - Likely Few mitotic - Likely 1     non-claret disjunctional ncd 7831 37 G 3 probe info Repeat 92.07 0.94% Yes   Yes + Unfocused spindle MTs Multipolar Multipolar - Clear Unfocused spindle MTs - Clear dim gtub in poles Unfocused spindle MTs - Clear Multipolar - Clear Few mitotic - Likely 1     Bub1 -GG Bub1 7838 37 G 6 probe info Repeat 82.35 2.82% Yes   No + Long spindle Long spindle - Likely Anaphase-like long spindles high circ2num More anaphase cells - Likely large spindle - Likely 1 2     RNA polymerase II 33kD subunit RpII33 7885 38 B 3 probe info Repeat 40.69 1.52% Yes   No + Long spindle Long spindle - Likely   Few mitotic - Likely large spindle - Likely 1   Ribosomal protein L32 RpL32 7939 38 E 3 probe info Repeat 51.74 0.64% Yes   No + Defective overall - Likely Defective overall   Defective overall - Likely Few mitotic - Clear 1     Eukaryotic initiation factor 1A eIF-1A 8053 39 C 4 probe info Repeat 45.65 0.55% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Likely 1     double parked (Cdt1 -GG) dup 8171 81 A 4 probe info Repeat 62.56 3.69% Yes   No +   high nuc num high circ2num DNA mass is tiny - Clear Few mitotic - Likely 1     PDGF- and VEGF-receptor related Pvr 8222 40 B 5 probe info Repeat 51.32 4.25% Yes   No + Short spindle Short spindle - Weak short spindles small spindle - Weak 1 2     TCP-1-zeta -GG CG8231 8231 40 B 9 probe info Repeat 83.86 4.72% Yes   No + Short spindle Short spindle - Weak short spindles small spindle - Weak 1 2 3     similar to chaperonin containing TCP1, subunit 8 (theta) CG8258 8258 40 D 3 probe info Repeat 57.53 7.17% Yes   Yes +   monopolar short spindles dim gtub in poles   1 2 3     Ana2 -GG CG8262 8262 105 D 10 probe info Repeat 67.43 9.49% Yes   No + Monastral bipolar - Weak Monastral bipolar   monastral bipolar - Weak This RNAi was accidentally repeated using 150D1 probe, and I marked "Anastral Likely" and "Monastral bipolar Weak". So, reproducible phenotypes. 1 2   Dynamitin Dmn 8269 79 G 6 probe info Repeat 172.00 9.65% Yes   Yes +     Although it is very hard to find out this subtle phenotype, I know I should not make an excuse. Shit!! Later I knew this is p50dynamitin, and indeed pole detachment is more frequently seen. I overlooked it. 1 2 3 4     &agr;-Tubulin at 67C &agr;Tub67C 8308 40 F 7 probe info Repeat 129.69 2.85% Yes   Yes + Dim MT Monopolar - Clear Monopolar Dim MT - Likely Short spindle Short spindle - Clear short spindles Dimmer MT staining - Likely Monopolar - Clear small spindle - Clear 1 2 3     Ribosomal protein S15 RpS15 8332 81 C 9 probe info Repeat 58.00 2.63% Yes   No + Short spindle Short spindle - Clear short spindles small spindle - Clear Few mitotic - Clear 1     TCP-1-eta homologue- GG CG8351 8351 40 H 10 probe info Repeat 101.90 6.80% Yes   Yes + Dim MT Dim MT - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Clear short spindles dim gtub in poles Monopolar - Clear small spindle - Clear Dimmer MT staining - Clear 1 2 3 4 5     dalmatian dmt 8374 127 G 7 probe info Repeat 126.64 3.46% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Clear large nuc area Large spindle - Likely Misalign - Clear Few mitotic - Weak 1 2   Small ribonucleoprotein Sm D3 SmD3 8427 41 D 6 probe info Repeat 33.81 2.22% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear large nuc area large spindle - Clear Misalign - Clear Few mitotic - Likely 1     T-complex Chaperonin 5 Cct5 8439 41 E 1 probe info Repeat 100.54 6.89% Yes   Yes + Dim MT Dim MT - Clear Short spindle Short spindle - Clear monopolar short spindles dim gtub in poles small spindle - Clear Dimmer MT staining - Clear 1 2 3 4 5     Calmodulin Cam 8472 80 E 4 probe info Repeat 152.19 9.13% Yes   No + Unfocused spindle MTs Long spindle Long spindle - Likely Monastral bipolar - Clear g-tub-like g-tub-like - Likely Unfocused spindle MTs - Clear Monastral bipolar long spindles large nuc area pole detach number pole detached half/cell Unfocused spindle MTs - Clear g-tub-like - Likely large spindle - Likely monastral bipolar - Clear 1 2 3 4   Kinesin-like protein at 3A Klp3A 8590 42 D 7 probe info Repeat 80.47 8.61% Yes   Yes + Misalign Misalign - Weak   Misalign - Weak 1 2 3 4     Eco1/deco -GG CG8598 8598 42 E 2 probe info Repeat 119.13 5.83% Yes   Yes + Misalign Misalign - Weak   Misalign - Weak 1 2 3 4 5     ribosomal protein L18e -GG CG8615 8615 42 F 4 probe info Repeat 78.82 0.70% Yes   No + Monopolar - Likely Monopolar Short spindle Short spindle - Clear   small spindle - Clear Monopolar - Likely Few mitotic - Clear 1     similar to eukaryotic translation initiation factor 3 subunit 4 CG8636 8636 42 G 1 probe info Repeat 97.29 0.74% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Likely 1     small nuclear ribonucleoprotein 70K snRNP70K 8749 43 C 12 probe info Repeat 48.46 0.52% Yes   No + Misalign Misalign - Likely   Thinner spindle - Weak Misalign - Likely Few mitotic - Likely 1     Dgt5 -GG CG8828 8828 107 D 6 probe info Repeat 166.52 10.28% Yes     + Long spindle Long spindle - Clear   Large spindle - Clear 1 2 3   Ribosomal protein S11 RpS11 8857 80 E 3 probe info Repeat 44.25 0.63% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Ribosomal protein S18 RpS18 8900 44 C 9 probe info Repeat 34.08 1.06% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Clear 1     Nuf2? - GG CG8902 8902 97 F 11 probe info Repeat 117.43 13.40% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear large nuc area high nuc num large spindle - Clear Misalign - Clear 1 2 3   Ribosomal protein S5 RpS5 8922 44 D 9 probe info Repeat 50.00 0.39% Yes   No + Short spindle Short spindle - Clear monopolar small spindle - Clear Few mitotic - Clear 1     TCP1, subunit 3 (gamma) -GG Cct&ggr; 8977 44 G 8 probe info Repeat 121.11 6.17% Yes   Yes + Dim MT Dim MT - Likely Short spindle Short spindle - Likely dim gtub in poles Dimmer MT staining - Likely small spindle - Likely 1 2 3     unknown -GG CG9028 9028 120 F 6 probe info Repeat 175.60 9.43% Yes     + Short spindle Short spindle - Likely short spindles Small spindle - Likely 1 2 3 4     Myb oncogene-like Myb 9045 45 B 9 probe info Repeat 58.61 6.75% Yes   No + Long spindle Long spindle - Clear Monastral bipolar - Clear Monastral bipolar   Few mitotic - Likely monastral bipolar - Clear large spindle - Clear 1 2   Eukaryotic initiation factor 4a eIF-4a 9075 45 C 9 probe info Repeat 71.80 2.30% Yes   No + Monopolar - Likely Monopolar Short spindle Short spindle - Likely   small spindle - Likely Monopolar - Likely Few mitotic - Clear 1     Kinesin-like protein at 61F Klp61F 9191 45 H 9 probe info Repeat 108.33 9.40% Yes   Yes + Monopolar - Clear Monopolar gtub area monopolar large nuc area high nuc num Monopolar - Clear 1 2 3 4     Grip128 -GG Grip128 9201 144 A 6 probe info Repeat 111.60 8.42% Yes   Yes + Long spindle - Weak Dim g-tub Long spindle Dim g-tub - Clear g-tub-like   g-tub-like - Weak Large spindle - Weak Dimmer g-tub - Clear 1 2     Glued Gl 9206 46 A 3 probe info Repeat 67.53 8.13% Yes   Yes + Pole detachment - Weak Pole detachment pole detached half/cell Pole detachment - Weak 1 2 3     similar to 60S ribosomal protein L24 CG9282 9282 46 E 1 probe info Repeat 188.41 1.30% Yes   No + Short spindle Short spindle - Clear short spindles Few mitotic - Likely small spindle - Clear 1 2     Pomp (Proteasome maturation factor UMP1 family member?) -GG CG9324 9324 78 H 4 probe info Repeat 72.12 2.72% Yes     + Dim g-tub Dim g-tub - Clear   Few mitotic - Clear Dimmer g-tub - Clear 1     Proteasome 29kD subunit Pros29 9327 46 G 6 probe info Repeat 108.82 4.09% Yes   No + Monastral bipolar - Weak Monastral bipolar monopolar Few mitotic - Likely monastral bipolar - Weak 1 2     &agr;-Tubulin at 85E &agr;Tub85E 9476 47 F 10 probe info Repeat 86.94 11.71% Yes   Yes + Dim MT Dim MT - Clear Monopolar - Clear Monopolar gtub area monopolar short spindles Monopolar - Clear Dimmer MT staining - Clear 1 2 3 4 5     Replication Protein A 70 RpA-70 9633 48 F 2 probe info Repeat 80.71 7.74% Yes   No + Long spindle - Weak Long spindle high nuc num large spindle - Weak Scattered chromatins - Likely 1 2 3     small nuclear ribonucleoprotein G -GG CG9742 9742 91 C 7 probe info Repeat 77.74 6.37% Yes   No + Long spindle - Weak Long spindle Misalign Misalign - Weak long spindles large nuc area large spindle - Weak Misalign - Weak 1 2       eIF3-S10 9805 49 E 12 probe info Repeat 100.84 2.47% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Likely 1 2     Ndc80 -GG CG9938 9938 50 C 6 probe info Repeat 100.94 5.56% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles Misalign - Clear large spindle - Clear Few mitotic - Weak Thinner spindle - Likely 1 2   heat shock construct of Qu eIF-2&agr; 9946 50 C 11 probe info Repeat 109.35 3.73% Yes   No +   short spindles   1 2     U2 small nuclear riboprotein auxiliary factor 50 U2af50 9998 50 E 10 probe info Repeat 104.20 3.96% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Clear   large spindle - Likely Misalign - Clear 1 2     Sas4 -GG CG10061 10061 50 H 6 probe info Repeat 175.31 5.82% Yes   No + Monastral bipolar - Likely Anastral Anastral - Weak Monastral bipolar   monastral bipolar - Likely anastral - Weak 1 2 3 4   SMC2 subunit of condensin -GG SMC2 10212 51 H 7 probe info Repeat 171.53 5.90% Yes   Yes + Misalign Misalign - Clear   Chromosome condensation is defective - Clear Misalign - Clear 1 2 3 4     Topoisomerase 2 Top2 10223 52 A 2 probe info Repeat 137.94 5.89% Yes   Yes +     I later knew this is Topo2. Looking these galleries again, I see some cells in which chromosome alignment or structure is indeed abnormal. I should have marked this as weak phenotype. Bummer! However it is also true that there are many normal-looking metaphase cells. Not too surprising that I initially missed this phenotype. 1 2 3 4     Dgrip71 -GG Grip71 10346 52 G 5 probe info Repeat 89.73 11.35% Yes   Yes + Dim g-tub Dim g-tub - Clear g-tub-like g-tub-like - Likely dim gtub in poles pole detach dist Weak g-tub staining - Clear g-tub-like - Likely 1 2 3 4     Heterogeneous nuclear ribonucleoprotein at 27C Hrb27C 10377 53 A 2 probe info Repeat 66.37 3.23% Yes   No + Defective overall - Likely Defective overall   Defective overall - Likely Few mitotic - Likely 1     male-specific lethal 1 msl-1 10385 78 E 12 probe info Repeat 233.44 8.55% Yes     + Misalign Misalign - Clear dim gtub in poles Misalign - Clear 1 2 3 4     MAK16-like protein RBM13 (RNA-binding motif protein 13-like) -GG CG10648 10648 54 E 2 probe info Repeat 133.07 4.13% Yes   No + Misalign Misalign - Weak Short spindle Short spindle - Likely short spindles Misalign - Weak small spindle - Likely 1 2 3     Ribosomal protein L30 RpL30 10652 78 F 10 probe info Repeat 99.72 1.68% Yes     + Monopolar - Likely Monopolar Short spindle Short spindle - Clear   Few mitotic - Clear Monopolar - Likely Small spindle - Clear 1     barren barr 10726 103 B 12 probe info Repeat 52.41 11.57% Yes Yes Yes + Long spindle Long spindle - Likely Misalign Misalign - Clear dim gtub in poles Large spindle - Likely Chromosome condensasion is defective - Clear Misalign - Clear 1 2 3     splicing factor 3a, subunit 2, 66kDa -GG CG10754 10754 55 A 1 probe info Repeat 41.75 2.95% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Likely   Misalign - Likely large spindle - Likely Few mitotic - Likely 1     Regulator of cyclin A1 Rca1 10800 97 F 4 probe info Repeat 44.20 5.96% Yes   No + Misalign Multipolar Misalign - Weak multipolar Multipolar - Likely Misalign - Weak Brighter MT staining - Clear Few mitotic - Clear 1     Eukaryotic-initiation-factor-4G eIF-4G 10811 55 B 10 probe info Repeat 105.20 7.69% Yes   No + Short spindle Short spindle - Weak short spindles small spindle - Weak 1 2 3 4     Kinesin-like protein at 67A Klp67A 10923 55 F 10 probe info Repeat 70.89 12.59% Yes   Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear Monastral bipolar - Likely Monastral bipolar monopolar long spindles large nuc area monastral bipolar - Likely Misalign - Clear large spindle - Clear 1 2 3 4     Ribosomal protein S6 RpS6 10944 55 G 7 probe info Repeat 50.43 0.53% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Clear 1     lethal (1) discs degenerate 4 (Dgrip91 -GG) l(1)dd4 10988 90 H 1 probe info Repeat 105.62 11.23% Yes   Yes + g-tub-like g-tub-like - Clear   g-tub-like - Clear 1 2 3 4     sin3a-associated protein sap130 -GG CG11006 11006 120 B 7 probe info Repeat 61.17 5.50% Yes     + Long spindle Long spindle - Clear   Few mitotic - Clear Large spindle - Clear 1     Ribosomal protein S4 RpS4 11276 57 D 10 probe info Repeat 64.45 0.59% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     gluon/SMC4 subunit of condensin -GG glu 11397 57 H 11 probe info Repeat 111.74 7.86% Yes   Yes + Long spindle Long spindle - Likely Misalign Misalign - Clear   large spindle - Likely Chromosome condensation is defective - Clear Misalign - Clear 1 2 3     unknown -GG CG11451 11451 124 B 5 probe info Repeat 119.28 1.14% Yes   No + Long spindle Long spindle - Likely Defective overall Misalign Misalign - Likely Defective overall - Clear   Defective overall - Clear Misalign - Likely large spindle - Likely Few mitotic - Clear 1     Ribosomal protein L6 RpL6 11522 89 D 5 probe info Repeat 39.83 1.53% Yes   No + Defective overall - Likely Defective overall   Defective overall - Likely Few mitotic - Clear 1     Dgt6 -GG CG11881 11881 136 D 12 probe info Repeat 165.24 12.10% Yes     + Dim g-tub Long spindle Long spindle - Clear Dim g-tub - Clear pole detach number Astral MTs look longer and more abundant. - Clear g-tub on the spindle is very dim. - Clear Dimmer g-tub - Clear Large spindle - Clear 1 2 3 4   splicing factor 3b, subunit 5 -GG CG11985 11985 86 E 2 probe info Repeat 46.25 1.20% Yes   No + Long spindle Long spindle - Likely   large spindle - Likely Few mitotic - Clear 1     proteasome beta 4 subunit -GG CG12000 12000 60 E 5 probe info Repeat 84.22 5.74% Yes   No + Monastral bipolar - Likely Monastral bipolar   monastral bipolar - Likely 1 2     Pre-mRNA splicing factor cwc15 -GG c12.1 12135 61 C 11 probe info Repeat 85.64 5.44% Yes   No + Misalign Misalign - Weak high nuc num Misalign - Weak 1 2 3     Inner centromere protein Incenp 12165 104 F 3 probe info Repeat 87.28 0.80% Yes   Yes + Defective overall - Weak Defective overall   Defective overall - Weak Chromatin structure is abnormal. - Clear Few mitotic - Clear 1     polo polo 12306 62 B 11 probe info Repeat 63.02 8.47% Yes   Yes + Anastral Anastral - Clear monopolar dim gtub in poles anastral - Clear I feel this is very similar to polo phenotype. - Clear Anastral spindle but pole is very well focused. 1 2 3     proteasome subunit beta 5-GG Pros&bgr;5 12323 62 C 6 probe info Repeat 57.77 5.75% Yes   No + Long spindle Long spindle - Clear Monastral bipolar - Weak Monastral bipolar dim gtub in poles monastral bipolar - Weak Few mitotic - Clear large spindle - Clear 1 2     ribosomal protein S15A -GG CG12324 12324 62 C 7 probe info Repeat 97.66 3.93% Yes   No + Short spindle Short spindle - Likely short spindles small spindle - Likely Few mitotic - Clear 1 2     nnp-1 protein (novel nuclear protein 1) -GG Nnp-1 12396 78 A 12 probe info Repeat 140.44 4.41% Yes     + Short spindle Short spindle - Clear   Small spindle - Clear Few mitotic - Weak 1 2     Ribosomal protein L28 RpL28 12740 83 E 4 probe info Repeat 42.78 2.21% Yes   No + Short spindle Short spindle - Likely   small spindle - Likely Few mitotic - Clear 1     ribosomal protein L21 -GG CG12775 12775 63 D 3 probe info Repeat 126.70 2.59% Yes   No + Short spindle Short spindle - Clear short spindles small spindle - Clear Few mitotic - Clear 1 2     Ana3 -GG CG13162 13162 107 B 12 probe info Repeat 94.16 9.30% Yes     + Monastral bipolar - Clear Anastral Anastral - Likely Monastral bipolar   Anastral - Likely Monastral bipolar - Clear 1 2     Splicing factor 3B, 14 kDa subunit - GG CG13298 13298 64 D 1 probe info Repeat 123.52 6.81% Yes   No + Long spindle Long spindle - Likely Misalign Misalign - Clear gtub area large nuc area high nuc num large spindle - Likely Misalign - Clear 1 2 3 4     centromere identifier/CENP-A- GG cid 13329 80 G 8 probe info Repeat 151.17 13.18% Yes   Yes + Long spindle Long spindle - Clear Misalign Misalign - Clear monopolar long spindles high nuc num high circ2num large spindle - Clear Misalign - Clear 1 2 3 4 5     Saccharomyces cerevisiae UAS construct a of Sotillos (RacGAP50C -GG) RacGAP50C 13345 64 D 12 probe info Repeat 96.29 9.24% Yes   No + Misalign Multipolar Misalign - Likely gtub area large nuc area g-tub dots are bigger - Likely Misalign - Likely This looks like a cytokinesis-defective cell (polyploid) - Likely Multipolar - Likely DNA masses are also bigger - Likely 1 2 3 4     Ribosomal protein S13 RpS13 13389 64 F 3 probe info Repeat 146.75 1.29% Yes   No + Multipolar Multipolar - Weak Short spindle Short spindle - Likely short spindles Multipolar - Weak small spindle - Likely Few mitotic - Clear 1     unknown -GG CG13550 13550 112 H 11 probe info Repeat 133.88 7.06% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles dim gtub in poles high nuc num high circ2num large spindle - Clear Misalign - Clear 1 2 3     RNA polymerase II 7.6 kDa polypeptide (RPB10) -GG Rpb10 13628 88 F 1 probe info Repeat 62.25 8.12% Yes   No + Dim g-tub Long spindle Long spindle - Clear Dim g-tub - Weak   large spindle - Clear Dimmer g-tub staining - Weak 1 2     Splicing factor -GG CG13900 13900 65 F 2 probe info Repeat 68.67 3.95% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles Few mitotic - Clear large spindle - Clear Misalign - Clear 1 2     unknown -GG CG14735 14735 128 F 12 probe info Repeat 122.78 5.86% Yes   No +   short spindles   1 2 3     unknown -GG CG14781 14781 138 D 4 probe info Repeat 117.68 10.71% Yes     + Monastral bipolar - Clear Monopolar - Clear Monopolar Short spindle Short spindle - Likely Monastral bipolar   Monastral bipolar - Clear Monopolar - Clear Small spindle - Likely 1 2 3   stubarista (Ribosomal protein SA -GG) sta 14792 67 E 6 probe info Repeat 55.00 1.11% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     unknown -GG l(2)08717 15095 81 F 6 probe info Repeat 151.89 9.14% Yes   No + Long spindle - Weak Long spindle Misalign Misalign - Clear long spindles large spindle - Weak Misalign - Clear 1 2 3 4   Ribosomal protein L27A RpL27A 15442 69 A 5 probe info Repeat 94.80 0.45% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Sas-6 -GG CG15524 15524 89 B 7 probe info Repeat 96.28 8.83% Yes   Yes +     This gene turned out to be Sas6, centriole duplication gene that should produce anastral or MAB spindles However, I still cannot see such defects here. The reason is that day4 is not long enough - I had the same problem before and had anastral phenotype at day 6 or 7. Therefore I mark this genes as a hit. 1 2 3   ribosomal protein S30 -GG CG15697 15697 69 E 10 probe info Repeat 76.76 0.77% Yes   No + Short spindle Short spindle - Clear   small spindle - Clear Few mitotic - Clear 1     Developmental embryonic B (similar to Small nuclear ribonucleoprotein F (snRNP-F) (Sm protein F) -GG) DebB 16792 70 D 1 probe info Repeat 66.53 4.05% Yes   No + Misalign Misalign - Likely   Misalign - Likely 1 2     splicing factor 3a, subunit 1 - GG CG16941 16941 70 G 7 probe info Repeat 76.63 0.51% Yes   No + Defective overall Defective overall - Clear large nuc area Defective overall - Clear Few mitotic - Clear 1     Dgt2 -GG CG16969 16969 100 B 7 probe info Repeat 68.67 13.40% Yes   No + Long spindle - Weak Dim g-tub Long spindle Dim g-tub - Clear dim gtub in poles large spindle - Weak Dimmer g-tub staining - Clear 1 2 3   CapG condensin -GG Cap-G 17054 71 B 6 probe info Repeat 98.73 4.34% Yes   Yes + Misalign Misalign - Likely   Misalign - Likely 1 2     Spd2 -GG CG17286 17286 122 B 9 probe info Repeat 80.92 7.59% Yes   No + Dim g-tub Misalign Misalign - Weak Monastral bipolar - Clear Anastral Anastral - Clear Dim g-tub - Clear Monastral bipolar   Misalign - Weak Dimmer g-tub signals - Clear anastral - Clear monastral bipolar - Clear 1 2     Ribosomal protein L10 - NS Qm 17521 85 H 7 probe info Repeat 63.89 0.43% Yes   No + Monopolar - Clear Monopolar   Monopolar - Clear Few mitotic - Clear 1     Dgt1 -GG CG18041 18041 74 C 10 probe info Repeat 188.39 3.04% Yes   No + Dim g-tub Dim g-tub - Weak dim gtub in poles Dimmer g-tub staining - Weak 1 2   Mis12? -GG CG18156 18156 117 E 1 probe info Repeat 74.45 15.35% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear long spindles high nuc num large spindle - Clear Misalign - Clear 1 2 3 4   unknown -GG CG18397 18397 78 F 5 probe info Repeat 178.36 11.21% Yes     +   short spindles   1 2 3 4     RNA polymerase II 19 kDa polypeptide -GG Rpb7 31155 87 C 11 probe info Repeat 75.84 4.27% Yes     + Misalign Misalign - Clear   Misalign - Clear 1     CENP-C -GG CG31258 31258 127 D 1 probe info Repeat 87.76 3.88% Yes   No + Long spindle Long spindle - Clear Misalign Misalign - Clear Pole detachment Pole detachment - Likely high circ2num Pole detachment - Likely Large spindle - Clear Few mitotic - Likely Misalign - Clear 1   chromosome bows (Mast/Orbit/CLASP -GG) chb 32435 85 F 8 probe info Repeat 138.72 10.01% Yes   Yes + Monopolar - Clear Monopolar monopolar I guess this is Mast/Orbit [CLASP] - Normal Monopolar - Clear 1 2 3 4   Tousled-like kinase tlk 32782 8 B 4 probe info Repeat 202.97 5.17% Yes   No + Dim g-tub Misalign Misalign - Clear Dim g-tub - Clear dim gtub in poles Misalign - Clear Dimmer g-tub - Clear 1 2 3     similar to calmodulin regulated spectrin-associated protein 1-like 1 -GG l(2)k07433 33130 81 D 8 probe info Repeat 111.58 12.42% Yes   No +   short spindles   1 2 3 4   Protein phosphatase 2A at 29B Pp2A-29B 33297 72 A 8 probe info Repeat 56.39 2.35% Yes   No + Defective overall - Likely Defective overall   Defective overall - Likely Few mitotic - Clear 1